Journal:

Article Title: Impaired Immune Responses and B-Cell Proliferation in Mice Lacking the Id3 Gene

doi:

Figure Lengend Snippet: (A) Splenocytes were stained with FITC-labeled anti-B220 monoclonal antibody after being loaded with Indo-1AM. B220+ B cells were examined for [Ca2+]i following stimulation (indicated by arrows) with rabbit anti-mouse CD19 or F(ab′)2 goat anti-mouse IgM. [Ca2+]i was determined as the ratio of Indo-1 fluorescence at 405 nm to 525 nm. The results are representative of three pairs of Id3+/+ and Id3−/− mice. (B) Expression levels of MHC class II antigen (Ia) in splenic B cells purified from Id3+/+ (top) and Id3−/− (bottom) mice after a 24-h culture in the absence (dashed lines) or presence (solid lines) of 10 μg of F(ab′)2 goat anti-mouse IgM per ml.

Article Snippet: Antibodies used in this study included biotinylated or FITC-conjugated anti-B220 (RA3-6B2; Caltag, Burlingame, Calif.), FITC-conjugated anti-CD43 (S7; PharMingen, San Diego, Calif.), biotinylated or FITC-conjugated goat anti-mouse IgM isotype-specific antibodies (Southern Biotechnology Associates, Inc., Birmingham, Ala.), biotinylated goat anti-mouse IgD isotype-specific antibodies (Southern Biotechnology Associates, Inc.), PE-conjugated anti-mouse I-Ab (AF6-120.1; PharMingen), anti-CD21/35 (7E9; provided by T. Tedder, Duke University, Durham, N.C.), PE-conjugated anti-CD23 (B3B4; PharMingen), PE-conjugated anti-CD4 (CT-CD4; Caltag), anti-CD8β (CT-CD8b; Caltag), PE-conjugated anti-CD5 (53-7.3; PharMingen), and PE-conjugated anti-MAC-1 (F4/80; Caltag).

Techniques: Staining, Labeling, Fluorescence, Expressing, Purification

Journal: Scientific Reports

Article Title: Engineering a reporter cell line to mimic the high oligomannose presenting surface immunoglobulin of follicular lymphoma B cells

doi: 10.1038/s41598-020-79862-2

Figure Lengend Snippet: FACS and fluorescence in vivo imaging. ( A ) Fluorescent microscopy of engineered BZ-mCherry cell line shows the clear red fluorescence of the reporter cell line (scale bar = 50um). ( B ) Ectopic murine BZ-mCherrry tumors are clearly visible by fluorescence in vivo imaging taken at Em = 620, Ex = 580, Bin = 4/4, Fnumber = f2, exposure = 0.5 s. ( C ) The potential use of the BZ-mCherry cell line for FACS based screening is also evident by the bright red fluorescence. ( D ) We also see a large shift in the mean fluorescent intensity for the BZ-mCherry cells compared to HEK when incubated with FITC-labeled anti-lambda probe.

Article Snippet: FITC labeled anti-lambda light chain (Cat# NB7551 Novus Biologicals) antibody was diluted 1:2000 ratio and blocked with 1% BSA in PBS buffer for 1 h at room temperature with rocking (50 rpm).

Techniques: Fluorescence, In Vivo Imaging, Microscopy, Incubation, Labeling

Journal: Bioengineering

Article Title: Systematic Review and Meta-Analysis of Remineralizing Agents: Outcomes on White Spot Lesions

doi: 10.3390/bioengineering12010093

Figure Lengend Snippet: Characteristics of included studies.

Article Snippet: Rana, N./2021/Punjab [ ] , Eighty extracted caries-free permanent premolars , Silverstone’s cariogenic solution for four weeks to induce artificial demineralization , Infiltrante de resina (Icon Infiltrant) (10) CPP-ACPF MI Paste Plus (10) NovaMin-fluoride toothpaste (10) , without treatment and washed with desionized water (10) , 14 days , Scanning electron microscopy with an energy dispersive X-ray analysis attachment (SEM-EDAX) testing Vickers Microhardness (VHN).

Techniques: Comparison, Purification, Light Microscopy, Cream, Fluorescence, Electron Microscopy